Pipettes

• Loading Pipettes
  • API Load Names
  • Loading Flex 1- and 8-Channel Pipettes
  • Loading a Flex 96-Channel Pipette
  • Loading OT-2 Pipettes
  • Adding Tip Racks
  • Adding Trash Containers
• Pipette Characteristics
  • Multi-Channel Movement
    • 8-Channel, 96-Well Plate Example
    • 8-Channel, 384-Well Plate Example
  • Pipette Flow Rates
    • Flex Pipette Flow Rates
    • OT-2 Pipette Flow Rates
  • OT-2 Pipette Generations
• Partial Tip Pickup
  • Nozzle Layout
  • Tip Rack Adapters
  • Tip Pickup and Conflicts
    • Using Column 12
    • Using Column 1
• Volume Modes

Opentrons pipettes are configurable devices used to move liquids throughout the working area during the execution of protocols. Flex and OT-2 each have their own pipettes, which are available for use in the Python API.

Pages in this section of the documentation cover:

• Loading pipettes into your protocol.

• Pipette characteristics, such as how fast they can move liquid and how they move around the deck.

• The partial tip pickup configuration for the Flex 96-Channel Pipette, which uses only 8 channels for pipetting. Full and partial tip pickup can be combined in a single protocol.

• The volume modes of Flex 50 µL pipettes, which must operate in low-volume mode to accurately dispense very small volumes of liquid.

For information about liquid handling, see Building Block Commands and Complex Commands.