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Temperature Module

The Temperature Module as seen from the top left. The top of the module has the heating and cooling surface and temperature display. The side has the power button, USB port, and power port.

Additional Documentation

For complete instructions on module installation and use, see the Temperature Module Instruction Manual.

Temperature Module features

Heating and cooling

The Opentrons Temperature Module GEN2 is a hot and cold plate module. It is often used in protocols that require heating, cooling, or temperature changes. The module can reach and maintain temperatures ranging from 4 °C to 95 °C within minutes, depending on the module's configuration and contents.

Thermal blocks

The Temperature Module uses interchangeable aluminum thermal blocks to help hold labware at temperature.

At the time of purchase, each Temperature Module includes your choice of one (1) thermal block. You can select a 24-well block, a 96-well block, a deep well block, or a flat-bottom block. The blocks hold 1.5 mL and 2.0 mL tubes, 96-well PCR plates, PCR strips, deep well plates, and flat bottom plates.

24-well aluminum thermal block for Temperature Module

24-well thermal block

96-well aluminum thermal block for Temperature Module

96-well thermal block

Deep well aluminum thermal block for Temperature Module

Deep well thermal block

Flat bottom aluminum thermal block for Temperature Module

Flat bottom thermal block for Flex

Software control

The Temperature Module is fully programmable in Protocol Designer and the Python Protocol API.

Outside of protocols, the Opentrons App can display the current status of the Temperature Module and can directly control the temperature of the surface plate.

Temperature Module specifications

Specification Details
Dimensions 194 × 90 × 84 mm (L/W/H)
Weight 1.5 kg
Module power
  • Input: 100–240 VAC, 50/60 Hz, 4.0 A
  • Output: 36 VDC, 6.1 A, 219.6 W max
Environmental conditions Indoor use only
Ambient temperature <22 °C (recommended for optimal cooling)
Relative humidity Up to 60%, non-condensing
Altitude Up to 2000 m above sea level
Pollution degree 2