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Pipette Installation, Removal, and Calibration

After the initial robot setup, your next step is to attach and calibrate a pipette. The OT-2 has two pipette mounts; each can hold a single-channel or multi-channel pipette. The Opentrons App provides detailed, illustrated instructions about attaching, removing, and calibrating these instruments. Follow these instructions to attach and calibrate a pipette or to remove it.

Attaching and calibrating

  1. Prepare your robot by removing any labware from the deck and clearing the working area. Give yourself room to work.

  2. In the Opentrons App, click the Devices tab and select the OT-2 you want to work with.

  3. In the Pipettes and Modules section for your device:

    • Select the left or right pipette mount.
    • Click the three-dot menu (⋮) and then click Attach pipette.

    Note

    The gantry will move towards the front of the deck and the mount will lower.

  4. In the Opentrons App, follow the on-screen instructions to connect the ribbon cable, secure the screws, and calibrate your pipette.

Detaching and removing

  1. Prepare your robot by removing any labware from the deck and clearing the working area. Give yourself room to work.

  2. In the Opentrons App, click the Devices tab and select the OT-2 you want to work with.

  3. In the Pipettes and Modules section for your device:

    • Click the three-dot menu (⋮) for the pipette you want to remove.
    • Click Detach pipette.

    Note

    The gantry will move towards the front of the deck and the mount will lower.

  4. In the Opentrons App, follow the on-screen instructions to remove the screws and detach the ribbon cable.